Fig. 6

Effect of CEFFE on hGCs A The identification of hGCs by FSHR expression. FSHR was labeled with green fluorescence, and the nucleus was labeled with blue fluorescence. Scale bar = 20 μm. B EdU assays were used to monitor cell proliferation (a) and quantified (b) (n = 4). Green fluorescence represents proliferative cells, and blue fluorescence represents the nuclei of all cells. Scale bar = 20 μm. C JC-1 fluorescence assays were used to monitor mitochondrial membrane potential (a) and quantified (b) (n = 4). Red indicates intact mitochondrial membrane potential, and green indicates damaged mitochondrial membrane potential. Scale bar = 50 μm. D TUNEL assays were used to monitor cell apoptosis (a) and quantified (b) (n = 3). Red fluorescence represented the apoptotic cells, and blue fluorescence represented the nucleus of all cells. Scale bar = 20 μm. Data are represented as the mean ± SD. ns: P > 0.05, compared with Control group; *P < 0.05, **P < 0.01, ***P < 0.001, compared with CTX group