Fig. 3
Comparison of single and triple implantations of DFAT cells for renal function, MPO-ANCA titer and expression of TSG-6 mRNA in kidney from SCG mice. SCG mice were infused with 105 DFAT cells through the posterior orbital venous plexus once at 8 weeks of age (DFAT × 1) or three times at 8, 9 and 10 weeks of age (DFAT × 3). Each mouse was killed at 12 weeks of age, its blood sampled to determine creatinine (A), blood urea nitrogen (BUN) (B) and titer of myeloperoxidase-antineutrophil cytoplasmic antibody (MPO-ANCA) (C), and its kidney removed to determine expression of TSG-6 mRNA (D). Total RNA was extracted from renal cortex and medulla from kidney, and mRNA expression of TSG-6 was determined by a real-time PCR system. Data are shown as the mean ± SEM (n = 4). *P < 0.05 in the indicated columns. SEM standard error of the mean; TSG-6 tumor necrosis factor-stimulated gene-6