Table 3 The sequence of primers
Gene | Sequence |
|---|---|
Runx2 | Forward: CTTCAAGGTTGTAGCCCTCG |
| Â | Reverse: TAGTTCTCATCATTCCCGGC |
ALP | Forward: CTAGTTCCTGGGAGATGGTA |
| Â | Reverse: GTGTTGTACGTCTTGGAGAGA |
OCN | Forward: CATGAGGACCCTCTCTCTGC |
| Â | Reverse: TGGACATGAAGGCTTTGTCA |
ATG5 | Forward: TGGGATTGCAAAATGACAGA |
| Â | Reverse: TTCCCCATCTTCAGGATCAA |
LC3 | Forward: TACCAAGGCAAAAAGGGACG |
| Â | Reverse: CCCCTGACACTGCTCTTCTAT |
P62 | Forward: AGCTGCCCTCAGCCCTCT |
| Â | Reverse: GGCTTCTCTTCCCTCC |
GAPDH | Forward: CCTCGTCTCATAGACAAGATGGT |
| Â | Reverse: GGGTAGAGTCATACTGGAACATG |
ATG14 | Forward: TGCCGAACAATGGGGACTAC |
| Â | Reverse: AGGCAGGGTTGTTATGCTCC |
miR-152-5P | Forward: GCCATTAGGTTCTGTGATACACTCC |
| Â | RT: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACagtcgg |
miR-20a-5p | Forward: GGGCCTAAAGTGCTTATAGTGCAG |
| Â | RT: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACctacct |
miR-125a-5p | Forward: GGTCCCTGAGACCCTTTAACCT |
| Â | RT: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACtcacag |
miR-146a-5p | Forward: GGGCTGAGAACTGAATTCCATG |
| Â | RT: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACaaccca |
miR-378-3p | Forward: GGGCACTGGACTTGGAGTCAG |
| Â | RT: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACccttct |
miR-100-5p | Forward: GGGAACCCGTAGATCCGAACT |
| Â | RT: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACcacaag |
miR-140-3p | Forward: GGGCTACCACAGGGTAGAACC |
| Â | RT: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACccgtgg |
miR-542-3p | Forward: GGGCCTGTGACAGATTGATAACT |
| Â | RT: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACtttcag |
miR-96-5P | Forward: GGGTTTGGCACTAGCACATTTT |
| Â | RT: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACagcaaa |
miR-185-5p | Forward: GCGTGGAGAGAAAGGCAGTT |
| Â | Reverse: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACtcagga |
U6 | Forward: CGCTTCGGCAGCACATATACTA |
| Â | RT: GGAACGCTTCACGAATTTGC |
Universe R | CCAGTGCAGGGTCCGAGGT |