Table 1 Primer sequence
Gene | Primer | TM (˚C) |
|---|---|---|
β-actin | F: GGCTGTATTCCCCTCCATCG R: CCAGTTGGTAACAATGCCATGT | 60 |
Gsk3b | F: GAGCCACTGATTACACGTCCAG R: CCAACTGATCCACACCACTGTC | 63 |
Wnt3a | F: CACCACCGTCAGCAACAGCC R: AGGAGCGTGTCACTGCGAAAG | 58 |
Akt1 | F: GCTTCTATGGTGCGGAGATTGT R: CGTCCTTGATCCCCTCCTTG | 63 |
MAPK1 or ERK2 | F: ATTGATATTTGGTCTGTGGGCT R: CCTGTTCCATGGCACCTTATTT | 63 |
MAPK3 or ERK1 | F: TCTGGTCTGTGGGCTGCATTC R: GGTAGTTTCGGGCCTTCATGT | 63 |
PI3K | F: AGTAACAGACTAGCCAGAGACAA R: TTGACAGACAGAAGCAATTTGGG | 60 |