Fig. 2

Somatic eIF5 is required for cyst cell differentiation and non-autonomously affects germ cell differentiation in adult testes. A Immunostaining of Vasa (red), 1B1 (green), Zfh1(red), and Eya (green) at the apex of testes of Ptc > GFP; tub-Gal80^ts and Ptc > GFP; eIF5 RNAi, tub-Gal80^ts at 0 days (29 °C). B Immunostaining of Vasa (red), 1B1 (green), Zfh1(red), and Eya (green) at the apex of testes of Ptc > GFP; tub-Gal80^ts and Ptc > GFP; eIF5 RNAi, tub-Gal80^ts at 9 days (29 °C). C Immunostaining of Vasa (red), 1B1 (green), Zfh1(red), and Eya (green) at the apex of testes of Ptc > GFP; tub-Gal80^ts and Ptc > GFP; eIF5 RNAi, tub-Gal80^ts at 15 days (29 °C). White double arrows represented regions of undifferentiated cells. DNA was stained with Hoechst (blue). Scale bars: 50 µm