Fig. 5

Transcription profiling of eIF5 in S2 cells. A Schematic diagram of the RNA-seq experiment in S2 cells. B Clustering heatmap of genes from the comparison between the NC and siRNA-1086 groups by Pearson correlation. The expression values were further normalized using the Z-score method. Red and green indicate high and low expression values, respectively. C Volcano plots based on -log₁₀FDR and log₂FC from the comparison of the NC and siRNA-1086 groups. D Circos plot of differentially expressed genes from the comparison of the NC and siRNA-1086 groups. From outer to inner, the tracks are expression heatmap, dots for FC, and dots for -logFDR values correspondingly. E–G Ten representative significantly enriched terms of GO: Biological process (E), GO: Molecular function (F) and KEGG pathways (G). Scaled colors correspond to -log₁₀FDR values, while the circle size is in proportion to the enrichment value. The number in each circle indicates the gene count. H–J Interaction network of selected genes and their associations with GO: Biological process (H), GO: Molecular function (I) and KEGG pathways (J). Red and green colors represent up- and downregulation, respectively. The overall size and border size are in proportion to the FC and − log₁₀FDR values correspondingly. Blue dashed lines and brown solid lines indicate gene-to-gene interactions and gene-to-function relationships, respectively