Fig. 1From: Melatonin in cryopreservation media improves transplantation efficiency of frozen–thawed spermatogonial stem cells into testes of azoospermic miceFlow cytometry to determine the purity percentage of isolated SSCs after two-step enzymatic digestion with α6-integrin, Thy1 and PLZF markers. M1: marker negative cells, M2: marker positive cells. a control; b α6-integrin positive; c Thy1 positive; d PLZF positive. e MTT analysis for assessment viability of frozen–thawed SSCs with different concentrations of melatonin. The results are presented as mean ± SD. ***p < 0.001Back to article page