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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Melatonin in cryopreservation media improves transplantation efficiency of frozen–thawed spermatogonial stem cells into testes of azoospermic mice

Fig. 1

Flow cytometry to determine the purity percentage of isolated SSCs after two-step enzymatic digestion with α6-integrin, Thy1 and PLZF markers. M1: marker negative cells, M2: marker positive cells. a control; b α6-integrin positive; c Thy1 positive; d PLZF positive. e MTT analysis for assessment viability of frozen–thawed SSCs with different concentrations of melatonin. The results are presented as mean ± SD. ***p < 0.001

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