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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Melatonin in cryopreservation media improves transplantation efficiency of frozen–thawed spermatogonial stem cells into testes of azoospermic mice

Fig. 6

Immunofluorescence staining for identifying differentiation marker (SCP3) 8 weeks after transplantation of frozen–thawed SSCs into recipient testes. Post-thawed SSCs labeled with DiI (red) and nuclei are stained in the recipient testes by DAPI (blue). Green-stained SSCs represent the expression of SCP3 marker in the control, Busulfan, Freeze and Freeze + Mel groups (a). The graph depicts the quantitative analysis for SCP3 marker in different groups (b). Scale bars = 50 μm. The results are presented as mean ± SD. Significance is indicated by **p < 0.01; ***p < 0.001

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