Fig. 5

Effect of different H₂O₂ concentrations on the antiapoptotic capacity of NPMSCs. a and b Effect of different H₂O₂ concentrations on the apoptosis ratio detected by flow cytometry and quantification (n = 3, *P < 0.05, **P < 0.01, one-way ANOVA followed by Bonferroni’s post hoc test). c and d Effect of 75 μM H₂O₂ pretreatment on the apoptosis ratio detected by flow cytometry and quantification when NPMSCs were subsequently exposed to 300 μM H₂O₂ (n = 3, *P < 0.05, **P < 0.01, one-way ANOVA followed by Bonferroni’s post hoc test. ^##P < 0.01 compared to the 300 μM H₂O₂ group). e and f Western blotting analysis estimating the effect of different H₂O₂ concentrations on Bcl-2, Bax, cleaved caspase-3, and cytochrome C expression and quantification (n = 3, *P < 0.05, **P < 0.01, one-way ANOVA followed by Bonferroni’s post hoc test). g and h Western blotting analysis estimating the effect of 75 μM H₂O₂ pretreatment on Bcl-2, Bax, cleaved caspase-3, and cytochrome C expression and quantification when NPMSCs were subsequently exposed to 300 μM H₂O₂ (n = 3, *P < 0.05, **P < 0.01, one-way ANOVA followed by Bonferroni’s post hoc test. ^#P < 0.05, ^##P < 0.01 compared to the 300 μM H₂O₂ group)