Fig. 5
Assessment of graft revascularization. A Angiogenesis shown in representative images of CD31-positive vessels in fragment-alone group, scaffold-fragment group, fragment-cell group, 3D-bioprinted engineering ovary group at 1 week after graft transplantation. Scale bar: 200 µm and 100 µm. B Data analysis of blood vessels density (number of blood vessels (nb) per mm.2) was performed in four treatment groups at 1 week and 4 weeks after transplantation. The significantly higher vessels density in 3D-bioprinted engineering ovary group from 1 to 4 weeks after transplantation indicated the more progressed and continuous vascular remodeling (* P < 0.05, ** P < 0.01, *** P < 0.001)