Fig. 2
From: Generation of an mESC model with a human hemophilia B nonsense mutation via CRISPR/Cas9 technology
Phenotypic analysis of mutant cell lines. a The mutant showed a typical mESC morphology (scale, 25 μm). b Karyotyping was performed by G-banding, which revealed diploid 40, XY. c Immunofluorescence (IF) staining was used to verify the expression of multipotent markers OCT4 and NANOG (scale, 50 μm). d qRT-PCR was used to verify the expression of pluripotency factor RNA in the mutant and compare it to the wild type. e, f EB differentiation verifies the mutant’s ability to differentiate into three layers in vitro when compared to the wild type. e A morphological diagram. f Expression of each layer after 6 days of differentiation, as well as pluripotency loss after 6 days of differentiation