Fig. 5

FSTL1 downregulation induces transcriptional reprogramming in MSCs. A Volcano plot showing DEGs in FSTL1^low MSCs compared to FSTL1^WT MSCs. The blue and red dots indicate downregulated and upregulated genes, respectively. Fold change > 1.5 and P value < 0.05) B Gene ontology (GO) analysis of the DEGs categorised as ‘Cellular component,’ ‘Molecular function,’ and ‘Biological process.’ C KEGG pathway enrichment analysis of the DEGs. The Y-axis represents KEGG terms, and the X-axis represents the rich factor. The colour of the bubble represents the enrichment significance, and the size of the bubble represents the number of DEGs. D The top downregulated enriched terms in KEGG pathway are presented as a bubble chart. E Representative differentially expressed genes (DEGs) between FSTL1^WT MSC and FSTL1^low MSC