Fig. 1
Diabetic plasma increases M1 cytokine expression, but nondiabetic plasma reduces M1 and increases M2 cytokine expression in Leprdb/db SVFs. SVFs were harvested from the adipose tissue of Lepr+/+ mice and treated with PBS or from the adipose tissue of Leprdb/db mice and treated with PBS or plasma from Leprdb/db or Lepr.+/+ mice in vitro. 3.5 h hours after treatment, cells were then collected and subjected to Q-PCR analysis for mRNA expression of TNFα, IL-6, IL-1β, IL-10, CCL2, and DPP4. N = 5/group. *p < 0.05, **p < 0.01, ***p < 0.001