Fig. 6
Nondiabetic plasma-treated SVFs decrease the expression of inflammatory meditators and suppress the activation of JNK and NFκB in the liver of Leprdb/db mice. SVFs were harvested from the adipose tissue of Leprdb/db mice and treated with plasma from Lepr+/+ or Leprdb/db mice. Lepr+/+ or Leprdb/db plasma-treated SVFs were injected into the inguinal WAT of Leprdb/db mice. Lepr+/+ and Lepr.db/db mice were also treated with PBS as controls. One week later, the liver was harvested and subjected to Q-PCR analysis for ICAM, FMO3, TNFα, iNOS, DPP4, and IL-6 mRNA expression (A), and western blot analysis for pJNK, JNK, pNFκB, NFκB, and DPP4 protein expression (B). N = 3–4/group. *p < 0.05; **p < 0.01