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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Bone morphogenetic protein 4 rescues the bone regenerative potential of old muscle-derived stem cells via regulation of cell cycle inhibitors

Fig. 1

Old MDSCs demonstrated reduced cell proliferation and multipotent differentiation when compared to young MDSCs. A Proliferation of young and old MDSCs measured by live-cell imaging system. Old MDSCs showed decreased cell numbers at 36 and 48 h after seeding (P = 0.031 and 0.004, respectively). B–C Myogenic differentiation. fMHC positive cells were significantly decreased in old MDSCs compared to young MDSCs after 7 days differentiation (P < 0.0001). fMHC stained red and nuclei stained blue. D–E Osteogenic differentiation. ALP positive staining percentage was significantly lower in old MDSC compared to young MDSCs (P = 0.0097). F–G Chondrogenic differentiation using pellet culture. Alcian Blue positive area was significantly lower in pellets formed by old MDSCs compared to that of young MDSCs (P = 0.0005). H–I Adipogenesis. Oil red positive (dark red) area percentage was significantly lower in old MDSCs group compared to that of young MDSCs group (P = 0.0502)

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Stem Cell Research & Therapy

ISSN: 1757-6512