Fig. 3

ADSC-EXO reverses profibrogenic phenotypes in CCl₄/DEN-induced liver fibrosis mice. A Collagen III (COL-III) and hyaluronic acid (HA) concentration in mice serum was measured by ELISA assay (n = 5/group). B Relative mRNA expression level of collagen deposition marker Collagen I and Collagen VI encoding gene Col1a1 and Col4a4 in mice liver tissue was determined by qRT-PCR analysis (4 replicates). C. Relative mRNA expression level of pro-fibronectin genes Acta2, Tgfb, Pdgfr, TIMP1 in mice liver tissue was determined by qRT-PCR analysis (4 replicates). D Protein expression level of α-SMA and Collagen I in mice liver tissue was detected by western blot analysis. (Each lane represents one mouse, 3 replicates.) GAPDH as internal reference. E Quantification statistic of (D). F Representative immunohistochemical staining images (IHC) of α-SMA in mice liver slices (scale bar, 100um; black arrows represent α-SMA positive area). G Quantification statistic of α-SMA⁺ area% in α-SMA IHC slices by Image J software analysis (5 replicates). H Representative IHC images of Collagen I in mice liver slices (scale bar, 100um; black arrows represent Collagen I positive area). I Quantification statistic of Collagen I ⁺ area% in Collagen I IHC slices by Image J software analysis (5 replicates). All data are shown as the mean ± SEM, *p < 0.05, **p < 0.01 comparing with corresponding controls by unpaired t test between two groups