Fig. 2

Verification of Dox-inducible overexpression of ETV2 in hDPSCs. a, b The optimal concentration of Dox was determined by qRT–PCR (a) and western blot (b). c-e Induction of ETV2 expression by Dox (100 ng/mL) at the mRNA and protein levels was measured by qRT–PCR (c), western blot (d), and immunofluorescence staining (e). f Detection of hDPSCs proliferation at days 1, 3, 5, and 7 by CCK-8 assay. Error bars, mean ± SD (n = 3). * P < 0.05. Scale bar: 50 μm