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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: ETV2 promotes osteogenic differentiation of human dental pulp stem cells through the ERK/MAPK and PI3K-Akt signaling pathways

Fig. 3

Transgenic ETV2 overexpression enhanced osteogenic differentiation of hDPSCs. a, b The expression of osteo-specific markers including ALP, COL1A1, and OSX was analyzed using qRT–PCR (a) and western blot (b). c Detection of osteogenic marker OSX at day 3 after osteogenic induction by immunocytochemistry and relative quantitative analysis. Scale bar: 50 μm. d ALP staining was conducted at days 7 and 14 of osteogenic differentiation. Scale bar: 200 μm. Histograms showing quantitative analysis of ALP activity. e The effects of ETV2 overexpression on calcium deposition were assessed by ARS staining at days 14 and 21 of osteogenic induction. Scale bar: 200 μm. Histograms showing quantitative data. Error bars, mean ± SD (n = 3). * P < 0.05

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Stem Cell Research & Therapy

ISSN: 1757-6512