Fig. 2
From: Knockdown of CDC20 promotes adipogenesis of bone marrow-derived stem cells by modulating β-catenin
Knockdown of CDC20 enhances adipogenic differentiation of hBMSCs. A Fluorescence micrographs showing the lentivirus transduction efficiency. Scale bar, 500 μm. B CDC20 mRNA expression in control shRNA (NC) and CDC20 knockdown (shCDC20-1, shCDC20-2) groups examined using qRT-PCR. (C, D) Oil red O staining C and quantification D of hBMSCs after adipogenic induction for 21 days. Scale bar, 100 μm. (E, F) Adipogenic regulators PPARG E and CEBPA F mRNA expression levels determined by qRT-PCR after adipogenic induction for 14 days. (G, H) Western blotting analysis G and quantification H of CDC20, PPARγ, C/EBPα, and the internal control GAPDH of negative control (NC) and CDC20 knockdown (shCDC20) cells in proliferation medium (PM) and adipogenic medium (AM) for 7 days. All data are presented as the mean ± SD (n = 3, **P < 0.01, ***P < 0.001)