Fig. 4
From: Knockdown of CDC20 promotes adipogenesis of bone marrow-derived stem cells by modulating β-catenin
Conditional knockout of Cdc20 promotes adipogenesis in the bone marrow of mice. A The design of the conditional deletion of the Cdc20 gene. B Images of PCR genotypes of control (Cdc20f/f) and conditional knockout (Sp7-Cre;Cdc20f/f) mice. C Immunofluorescence of the relative expression of CDC20 in bone sections of control (Cdc20f/f) and conditional knockout (Sp7-Cre;Cdc20f/f) mice. Scale bar, 100 μm. D H&E staining of paraffin sections from femurs and tibiae of 6-week-old control (Cdc20f/f) and conditional knockout (Sp7-Cre;Cdc20f/f) mice. Scale bar, 100 μm. E Quantification of fat cell density and fat tissue fraction based on the H&E staining of Cdc20f/f and Sp7-Cre;Cdc20f/f mice. All data are presented as the mean ± SD (n = 3, **P < 0.01, ***P < 0.001)