Fig. 5
From: Knockdown of CDC20 promotes adipogenesis of bone marrow-derived stem cells by modulating β-catenin
Conditional knockout of Cdc20 increases adipogenic differentiation of mBMSCs. (A, B) The knockout efficiency of Cdc20 in BMSCs of Cdc20f/f and Sp7-Cre;Cdc20f/f mice examined by qRT-PCR A and western blotting analysis B. (C, D) Oil red O staining C and quantification D at day 21 after adipogenic induction of mBMSCs from Cdc20f/f and Sp7-Cre;Cdc20f/f mice. Scale bar, 100 μm. (E, F) Adipogenic markers Pparg E and Cebpa F mRNA expressions determined by qRT-PCR after adipogenic induction for 14 days of mBMSCs from Cdc20f/f and Sp7-Cre;Cdc20f/f mice. All data are presented as the mean ± SD (n = 3, ***P < 0.001)