Table 1 Genotyping primers
From: Knockdown of CDC20 promotes adipogenesis of bone marrow-derived stem cells by modulating β-catenin
Cdc20 5′loxP-F | CCTAAACTATGTGGAGTTCAAGGCCA | WT:202 bp |
|---|---|---|
Cdc20 5′loxP-R | AGGATCTAGGATCTAGGTGACTCCC | Mut:321 bp |
Cdc20 3′loxP-F | GAAGCAGCTCCTGTCTTGGAGTTGT | WT:405 bp |
Cdc20 3′loxP-R | CCACAGCCTGGGTGGAATGGATAAA | Mut:490 bp |
Sp7-Cre WT-F | TACCAGAAGCGACCACTTGAGC | 263 bp |
Sp7-Cre WT-R | CGCCAAGAGAGCCTGGCAAG | 263 bp |
Sp7-Cre Mut-F | TACCAGAAGCGACCACTTGAGC | 445 bp |
Sp7-Cre Mut-R | GCACACAGACAGGAGCATCTTC | 445 bp |