Table 2 Sequences of RNA and DNA oligonucleotides
From: Knockdown of CDC20 promotes adipogenesis of bone marrow-derived stem cells by modulating β-catenin
Sense strand/ sense primer (5′-3′) | Antisense strand/ antisense primer (5′-3′) | |
|---|---|---|
Primers | ||
GAPDH | AAGGAGTAAGACCCCTGGACCA | GCAACTGTGAGCAGGGGAGATT |
PPARG | GAGGAGCCTAAGGTAAGGAG | GTCATTTCGTTAAAGGCTGA |
CEBPA | GGGCCAGGTCACATTTGTAAA | AGTAAGTCACCCCCTTAGGGTAAGA |
FABP4 | CCTTAGATGGGGGTGTCCTGGT | GCCTTTCATGACGCATTCCACC |
CDC20 | TGTGTGGCCTAGTGCTCCTG | ACACCATGCTACGGCCTTGA |
Gapdh | CAGGAGAGTGTTTCCTCGTCC | TGAAGGGGTCGTTGATGGCA |
Cdc20 | CTCAAAGGACACACAGCACGG | CGCCACAACCGTAGAGTCTCA |
Pparg | ACGGGCTGAGGAGAAGTCAC | TCACCGCTTCTTTCAAATCTTGTCT |
Cebpa | ATTCCTGCTTCCTCTCGGGC | ACTGCCGGGATCTCAGCTTC |
shRNA | ||
Control | TTCTCCGAACGTGTCACGT | |
shCDC20-1 | GCACTGGACAACAGTGTGTAC | |
shCDC20-2 | GCAGAAACGGCTTCGAAATAT | |