Fig. 8

ADAM19 overexpression reverses the effect of miR-335-5p on epithelial–myofibroblast transdifferentiation (EMT) and inflammation in TGF-β1-induced HK-2 cells. A ADAM19 protein levels were measured by western blotting in TGF-β1-treated HK-2 cells after co-transfection of the miR-335-5p mimic and ov-ADAM19 (n = 3). B The shape of TGF-β1-treated HK-2 cells changed from a normal HK-2 cell morphology to a long spindle shape after co-transfection of the miR-335-5p mimic and ov-ADAM19 in TGF-β1-treated HK-2 cells (n = 3). C The levels of TNF-α, IL-6, and IL-1β were increased whereas the levels of IL-4 and IL-10 were decreased after co-transfection of the miR-335-5p mimic and ov-ADAM19 in TGF-β1-treated HK-2 cells (n = 3). D The protein levels of collagen I, collagen III, α-SMA, vimentin, and N-cadherin in TGF-β1-induced HK-2 cells were increased, whereas E-cadherin protein level was decreased after co-transfection of the miR-335-5p mimic and ov-ADAM19 in TGF-β1-treated HK-2 cells (n = 3). E The statistical results of protein expression (n = 3). All statistical analyses were performed by one-way ANOVA, followed by Tukey’s multiple comparisons post hoc test. *P < 0.05