Fig. 3

RUNX1T1 regulates the cell fate choice of intestinal progenitor cells in collaboration with Notch and ATOH1 (A). The activation of Notch steers differentiation towards enterocytes, while its inactivation represses the self-renewal program of stem cells and de-represses ATOH1 and RUNX1T1, promoting differentiation towards secretory lineages (B). When Notch is interfered with by DAPT, ATOH1 is de-repressed and undergoes rapid upregulation, followed by increased RUNX1T1 expression. Meanwhile, the intestinal stem cells tend to differentiate into secretory cells (C)