Fig. 3

Effects of HA on the migration, cell size and colony formation capabilities of LESCs. TKE2 cells were maintained until confluence and, thereafter a scratch made through the center of the culture dish using a pipette tip, as previously shown [87] (A). A representative image of TKE2 cells accumulating in clusters instead of evenly distributing throughout the culture dish when maintained on PLL/HA coated dishes (B). The colony formation capabilities of TKE2 cells were assayed on differently coated dishes compared to uncoated controls and in the presence or not of Hyalase and 4MU (C). The number of holoclones that were larger than 1000 μm were counted (C). The cell area (D) and cell circularity (E and F) was quantified for hLESCs maintained on differently coated dishes. The colony formation capabilities of hLESCs were assayed when maintained on differently coated dishes in the presence (G) or absence of 3T3 cells (H). The colony formation capabilities (I) and cell area (J) of hLESCs were assayed after being maintained on ColIV or on ColIV followed by PLL/HA. Representative images captured using an EVOS microscope evidencing the morphology of hLESCs maintained of the differently coated dishes (K). *Represents p ≤ 0.05, scale bar represents 1000 μm