Fig. 5
Propofol increases Src expression via GABAAR. A Western blotting for p-Src and Src in wild-type or GABAAR β3/δ knockout (KO) U118-derived GSCs treated with propofol (n = 5 in each group). GAPDH was used as a loading control. B The mRNA levels of Src in U118-derived GSCs treated with or without propofol were analyzed by RT-PCR (n = 5 in each group). β-actin was used as the loading control. C CHX chase assay indicates that the half-life of Src protein was markedly prolonged, by western blot analysis, with the treatment with muscimol or propofol compared to control conditions. GAPDH was used as the loading control. D Western blot analysis of Src ubiquitylation in U118-derived GSCs following the overexpression of Src-Flag or control vector and treatment with propofol or control condition. Src was immunoprecipitated with anti-Flag and immunoblotted with an anti-HA antibody. E Schematic representation demonstrating that propofol can enhance the stem-like properties of gliomas through the interaction of the GABAAR-Src-ZDHCH5-EZH2 signaling axis. Ctrl, control; p-Src, phosphorylated Src; and CHX, cycloheximide