Table 1 Choice of culture media investigated for BDAC sourcing
Culture media | Short form | Xeno free/serum free |
|---|---|---|
Dulbecco's modified Eagle medium with 10% fetal bovine serum (FBS) and Penicillin–Streptomycin (P/S), Standard media (established protocol) Gibco (DMEM, cat.# 31600092; FBS, cat. #16000044; P/S, cat. #15140-122; Life Technologies, Grand Island, NY, USA) | DMEM/FBS | – |
CnT-Prime MSC Proliferation Medium, Xeno-Free CELLnTEC Advanced Cell Systems AG (cat. #CnT-PR-MSC-XF; Stauffacherstrasse, Bern, Switzerland) | CnT | Xeno free |
Human Stem X Vivo serum-free MSC expansion media R&D systems (cat. #CCM014; Minneapolis, USA) | StemXVivo | Serum free |
MSC NutriStem XF medium (supplemented with PLTGold Human Platelet Lysate, research grade) Sartorius AG (cat. #05-200-1A, #PLTGOLD100R; Goettingen, Germany) | NutriStem | Xeno free |
TheraPEAK Mesenchymal Stem Cell Growth Medium Lonza (cat. #BEBP18-936; Basel, Switzerland) | TheraPEAK | Chemically defined |
Mesenchymal Stem Cell growth medium DXF PromoCell (cat. #C-28019; Heidelberg, Germany) | DXF | Xeno free, serum free |