Fig. 7

Exosomal miR-146a-5p regulates Irak1 and Traf6 by targeting their mRNA 3’-UTR. a Predicted binding sites of miR-146a-5p on Irak1 and Traf6 mRNAs. b Luciferase reporter assay of predicted binding sites of miR-146a-5p in HEK293 cells. c d Irak1 and Traf6 gene expressions in astrocytes transfected with different doses of miR-146a-5p mimics and (e f) inhibitors assayed by western blot and QRT-PCR. Data above are represented as mean ± SD. *, p < 0.05; **, p < 0.01. #, p > 0.05. WT wild type, MUT mutant, QRT-PCR quantitative real-time polymerase chain reaction, Traf6 TNF receptor associated factor 6, Irak1 interleukin 1 receptor associated kinase 1