Fig. 9

miR-146a-5p-modified exosomes reduced toxicity of neurotoxic astrocytes by targeting Traf 6/Irak1/NFκB pathway. a Representative images and quantification of western blots for C3, Lcn2 and critical mediators of NFκB signaling pathway in control astrocytes, neurotoxic astrocytes (Mix), ExoN/ExoOE treated neurotoxic astrocytes and PDTC treated neurotoxic astrocytes. b Representative images and quantification of western blots of p-p65 in nucleus of astrocytes with different administrations. c, d Immunostaining images of p-p65 in nucleus and quantification of the mean immunofluorescence intensity of total p-p65 per cell and nuclear p-p65 per cell. Scale bar = 50 μm. Data above are represented as mean ± SD. *, p < 0.05; **, p < 0.01; #, p > 0.05. PDTC ammonium pyrrolidine dithiocarbamate, P-p65 phospho-NFκB p65, P-ikb phospho-ikb