Fig. 4

Oct4 accelerates cMSCs to switch toward an angiogenesis phenotype. A Angiogenesis, inflammation, and fibrosis of cMSCs subjected to transfection of oec-Myc or sic-Myc in the absence or presence of oeOct4, as determined using immunofluorescence with anti-vWF (red)/CD31 (green), CD80 (red)/CD11b (green), and collagen I (red)/vimentin (green), respectively. The nuclei were counterstained with DAPI (blue). B C D Double-positive cells in angiogenesis, inflammatory, or fibrosis proteins were expressed as a percentage of vWF⁺CD31⁺ B, CD80⁺CD11b⁺ C, or collagen I⁺vimentin⁺ D relative to all cMSCs from A. All data are the means ± SEM; statistical significance was evaluated using the unpaired two-tailed Student’s t test with Welch’s correction. Comparison of oeOct4⁺oec-Myc⁺ is indicated as follows: ^*P < 0.05, ^**P < 0.001, ^***P < 0.001. Comparison of oec-Myc or sic-Myc + oeOct4 is indicated in the same manner but using the symbol “#,” or “†,” respectively; ^###P < 0.001; ^††P < 0.001, ^†††P < 0.001. E Immunoblotting with anti-vWF/CD31, CD80/CD11b, and collagen I/vimentin in cMSCs culture system with transfection of oec-Myc or sic-Myc in the absence or presence of oeOct4. DAPI, 4′, 6-diamidino-2-phenylindole