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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Stable expression of a truncated TLX variant drives differentiation of induced pluripotent stem cells into self-renewing neural stem cells for production of extracellular vesicles

Fig. 1

Construction of the iPSCTLX lines. A Illustrations of TLX constructs. B Flow cytometric analysis of TLX expressions in iPSCTLX−FL/TP lines. C Detection of NR2E1 expression in iPSCTLX−FL/TP lines by qRT-PCR. D Morphology of iPSCsTLX−FL/TP. E Analysis of EGFP expression in different passages of iPSCsTLX−FL/TP by flow cytometry. The expression level and stability of the EGFP gene was not controlled due to the independent expressions of EGFP and the puromycin-resistant genes, hence the two populations of EGFP+ cells observed in flow cytometry. F Detection of NR2E1 expression in different passages of iPSCsTLX−FL/TP by qRT-PCR. G Proliferation curves of iPSCTLX−FL/TP lines. H qRT-PCR analysis of OCT4, SOX2 and NANOG expressions in iPSCsTLX−FL/TP. The data are representative of three independent experiments and expressed as the means ± SD, ****p < 0.0001, n. s. means no significant difference

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Stem Cell Research & Therapy

ISSN: 1757-6512