Fig. 4
Long-term passaging of iNSCsTLX−FL/TP. A Proliferations of iNSCsCMV−TLX−FL/TP and iNSCsEF1a−TLX−FL/TP. B Expressions of Nestin, Sox2, Vimentin and Pax6 in different passages of iNSCsCMV−TLX−FL/TP and iNSCsEF1a−TLX−FL/TP detected by flow cytometry. C Karyotype identification showed that the chromosomal integrity of the iNSCTLX−TP lines was unaffected. D Expression of Flag in different passages of iNSCsEF1a−TLX−FL/TP detected by immunoblotting. E qRT-PCR analysis of expressions of PAX6, PTEN, P21 and NR2E1 genes in iNSCsEF1a−TLX−FL/TP. F Protein expressions of Flag, PTEN and GAPDH determined by immunoblotting. The data are representative of three independent experiments and expressed as the means ± SD, **p < 0.01, ***p < 0.001