Fig. 6
Purification and characterization of iNSC-EVs. A Scheme of purification steps of iNSC-EVs. B Nanoflow cytometry (nanoFCM) results of iNSC-EVs (blue), iNSC-EVs treated with Triton X-100 (red) and Triton (green) after the TFF step, fractions of DGUC (7–11) and QA (D). C Concentration of DGUC fraction detected by nanoFCM. TEM results of iNSC-EVs after the TFF step (E), QA (G) and a fraction (1–6) or fraction (7–11) of DGUC (H). I Immunoblotting of iNSCsTLX−TP or purified iNSC-EVs for Flag, CD63 and CD81 expressions