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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Knockdown of FOXA1 enhances the osteogenic differentiation of human bone marrow mesenchymal stem cells partly via activation of the ERK1/2 signalling pathway

Fig. 5

Stain effects of FOXA1 overexpression on osteogenic differentiation and proliferation by FOXA1 overexpression of hBMSCs. A, B overexpression of FOXA1 significantly reduced hBMSC ALP activity (after 7 days of osteogenesis) and calcium deposits. (after 14 days of osteogenesis) in ALP and ARS staining. Scale bars = 50 μm. C, D Relative expression of osteo-specific proteins (COL1A1 and RUNX2) determined by IF on day 5 of osteogenesis. Nuclei were counterstained with DAPI. Scale bars = 200 μm. E The CCK-8 assay revealed that hBMSCs proliferation was not affected by FOXA1 overexpression (FOXA1-OE). All data are means ± SDs (n = 3). *p < 0.05, **p < 0.01, and ***p < 0.001 versus the control group

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Stem Cell Research & Therapy

ISSN: 1757-6512