Fig. 6

Enhanced osteogenic differentiation of hBMSCs due to FOXA1 knockdown was partially reduced by the addition of ERK1/2 signalling inhibitors. A, B Increased expression of osteo-specific proteins COL1A1, RUNX2, SP7 (normalised to that of GAPDH) and the level of p-ERK1/2 (normalised to that of ERK) induced by FOXA1 knockdown were almost suppressed by PD98059. C, D Increased ALP activity and mineralisation of hBMSCs by FOXA1 knockdown were attenuated by PD98059. Scale bars = 100 μm. E, F The inhibitor almost completely suppressed the increased RUNX2 and COL1A1 proteins detected by IF. All data are means ± SDs (n = 3). *p < 0.05, **p < 0.01, and ***p < 0.001 versus the control group