Fig. 4 

iFC-based interactive transcripts modulate the proliferation–maturation axis of hiPSC-CMs TROPO-GFP in vitro at D30. A hiPSC-CM proliferation estimated using the EdU assay and immunofluorescence-based detection of Ki67⁺. Results were plotted as the percentage calculated by the number of cTnI cells with EdU/Ki67 positive nucleus versus all the cTnI positive nucleus n = 4 independent differentiation experiments. B Structural shift analysis based on image mean fluorescence intensity of ssTnI (TNNI gene) and cTnI (TNNI3) for each experimental group n = 4–6 independent differentiation experiments. C Cell morphology analysis based on area and eccentricity measurements. D Representative images of cells treated with selected miRNAs and subjected to immunofluorescence for α-Actinin (Scale bar = 40 μm). Order, dispersion, and spacing of α-Actinin filaments were calculated on MorphoScript, a MATLAB-based image analysis toolbox (N = 91–142 cells per generated from two independent transient transfections originating from the same differentiation). Dashed lines represent the median of control cells in each graph. Statistical significance is represented as * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001