Fig. 6

MiR-124-3p and miR-512-3p regulate the dynamics of SR-mediated Ca²⁺ release and reuptake, and the action potential on hiPSC-CMs GJA1-GFP at D30. A Relative Levels of Intracellular Calcium in Ctrl, miR-124-3p-treated cells and miR-512-3p-treated cells. B Effects of SERCA inhibitor thapsigargin (5 µm) on [Ca²⁺]I in three-point: Basal (20 s), 200–220 s, and 400 ~ 440 s in spontaneously beating cells. Normalized frequency (%) (displayed as percentage decreased versus baseline condition) (N = 4 independent differentiation experiments). C Representative Ca²⁺ transients in spontaneously beating Ctrl, miR-124-3p and miR-512-3p before and after the addition of caffeine (10 mM) (N = 4 independent differentiation experiments). D Relative levels of Ca²⁺ storage after caffeine addition were normalized to those from the same cell under the basal condition (N = 4 independent differentiation experiments). E Box plot of Peak amplitude (s) in Ctrl and miR-124-3p-treated cells. F Box plot of decay time 50% (ms) in Ctrl and miR-124-3p-treated cells (N = 5 independent differentiation experiments). G Expression levels of gene in Ctrl versus miR-124-3p-treated cells (N = 4 independent differentiation experiments). Statistical significance treatment vs control (Ctrl) are represented as * p < 0.05, ** p < 0.001