Fig. 6
hESC-derived RPE required a 1-week exponential stage upon passaging. A Representative bright-field images showed the morphology of hESC-derived RPE cells at different time points in culture. B The growth curve of hESC-derived RPE was plotted as the cell number versus culture time. The data were fitted with an exponential-plateau model with R2 = 0.9404. C Representative fluorescent images of EdU-labeled RPE cells at different time points in culture. The nuclei were counter-stained with DAPI. Scale bars: 100 µm. D The quantification of the percentage of EdU-labeled RPE cells at different time points in culture. E The recovery, viability, and attachment rates were measured upon thawing of hESC-derived RPE cells frozen at indicated time points. n = 3 independent experiments; Data are represented as mean ± SD. Statistical differences are evaluated with one-way ANOVA with Tukey's post hoc test. *P < 0.05