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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Retinoic acid delays initial photoreceptor differentiation and results in a highly structured mature retinal organoid

Fig. 2

Protocol-dependent temporal expression of photoreceptor markers. Pools of 25–30 retinal organoids were collected at different differentiation time points (D35, D60, D90, D120, D150 and D225) and relative gene expression was measured by qPCR for the photoreceptor markers: CRX (A), NR2E3 (B), NRL (C), RCVRN (D) GRK1 (E), RHO (F) ARR3 (G) and OPN1MW (H). Grey lines represent Protocol 2 and black lines Protocol 3. Light grey boxes indicate window of RA treatment for Protocol 3. Data are normalised to the housekeeping gene GAPDH and represented as mean ± SEM, n = 3

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Stem Cell Research & Therapy

ISSN: 1757-6512