Fig. 2

Hsa_circ_0001485 promoted osteogenic differentiation in the osteogenic hFOB 1.19 cells. A qPCR evaluated efficiency of hsa_circ_0001485-targeted siRNA (si-circ1485) after 48 h siRNA transfection in hFOB 1.19 cells. B Alizarin red (AR) and ALP staining of osteogenic hFOB 1.19 cells transfected with si-circ1485. For AR, magnification, 100 ×, scale bar = 200 μm; for ALP, magnification, 200 ×, scale bar = 100 μm. C ALP activity assay in osteogenic hFOB 1.19 cells transfected with si-circ1485. D, E The protein levels of RUNX2, OPN, and OCN were detected via western blot after transfection with si-circ1485 and quantification by densitometric analysis. F Osteogenic hFOB 1.19 cells overexpressing hsa_circ_0001485 (OE-circ1485) were constructed by plasmid and confirmed by qPCR assay. G AR staining and ALP staining of osteogenic hFOB 1.19 cells transfected with OE-circ1485. For AR, magnification, 100 × , scale bar = 200 μm; for ALP, magnification, 200 ×, scale bar = 100 μm. . H ALP activity assay in cells transfected with OE-circ1485. I, J After transfection with OE-circ1485 and quantification by densitometric analysis, Western blot detected RUNX2, OPN, and OCN levels. The results are representative data from three replicates, and the data mean ± SD (*P < 0.05; **P < 0.01; ***P < 0.001)