Fig. 4

The IFN-γ- and KYNA-primed hADSCs can promoted the polarization of M2 macrophages. (A) Rat with the TNBS-induced colon fibrosis were killed on day 46 to harvest the spleen, mLN (n = 3). CD45RA⁺ B cells, CD3⁺ T cells, CD4⁺ T cells, CD8⁺ T cells, CD43⁺His48^Int−Lo monocytes and CD43⁺His48^Hi neutrophils in spleens of each group were detected by flow cytometry and statistical (Int-Lo means interval lower positive cells, Hi means strongly positive cells); (B) Blood routine analysis of WBC, lymphocyte, monocytes, and neutrophil in peripheral blood after primed hADSCs treatment (n = 3); (C) Analysis of macrophage subsets change. The infiltration of CD86⁺M1 macrophages and CD206⁺M2 macrophages in spleens of each group was detected; the following statistical figure shows the analysis of CD86⁺M1 and CD206⁺M2 macrophages in the CD68⁺ cell population (n = 3); data are represented as mean ± SEM