Fig. 5
hADSCs ameliorated TNBS-induced colitis through an IDO-1-dependent manner. (A) Experimental layout of hADSCs and hADSCsIDO−1−KO transplantation in the TNBS-induced acute colitis in rats; (B) Endoscopy observed and statistical on the 7th day (n = 5); (C) Body weight change (n = 5); (D) DAI score (n = 5); (E) Rats were killed at the 10th day, a. anatomic observation (n = 5). b. length of the colon was counted (n = 5). c. the lesions of the colon were observed (n = 5); (F) H&E staining was performed to observe the structural integrity of intestinal and the infiltration of immune cells in each group and score (Magnification 10X; Scale bar = 100 μm; n = 5); (G) qPCR detection mRNA expression changes of inflammatory cytokines IL-1β, TNF-α and anti-inflammatory molecule IL-10 (n = 5). Data are represented as mean ± SEM