Fig. 3

TD induces gradual CpG demethylation. A Heat map of top 52 differentially methylated CpGs with delta =  > 0.15 from donor B, at day 2 and day 6 after initiation of TD (adjusted P < 0.05) (Pearson linear correlation). B Liver cells were induced to transdifferentiate for 6 days. Gene expression analysis was performed 2–6 days post trans-differentiation of genes that were demethylated by the treatment: A SDC1 (Syndecan 1), B USP47 (Ubiquitin Specific Peptidase 47), C JmjD1C, (Jumonji Domain Containing 1C) D SULT1C4, E UBA5 (Ubiquitin Like Modifier Activating Enzyme 5), and F GCLC (Glutamate-Cysteine Ligase Catalytic Subunit). Results are the average of RNA extracted from 3 different donors