Fig. 8

hsa_circ_004658/hsa-miR-1200 axis suppresses the Wnt/β-catenin signalling pathway during hepatic specification. A The constructed psiCHECK2 plasmid for dual–luciferase reporter assay. B Three putative binding sites between hsa_circ_004658 and miR-1200. C The sequences of miR-1200 mimics. D Verification of miR-1200 as a target miRNA of hsa_circ_004658 by the dual–luciferase reporter assay. E Fluorescence intensities of the markers (β-catenin, p-β-catenin and TCF4) of the Wnt/β-catenin signalling pathway among the miR-1200 mimics, mimics NC and control groups. F Relative optical density analysed by Image J. Western blotting experiments were repeated three times. *p < 0.05, **p < 0.01; ns, not significant