Fig. 4

DH treatment specifically impaired erythroid maturation and enucleation from erythroblasts to erythrocytes. A Schematic of DH treatment at various time points during erythroid differentiation. B Representative of erythroid cell morphology of differentiated cells at day 18 following treatment with DH at the various time points described above, showing mature erythrocytes (red arrows) and erythroblasts (black arrows). C Percentages of mature erythrocytes and erythroblasts after timed DH treatment during differentiation at the terminal differentiation stage (day 18). At least 500 cells were counted in each group (n = 4). Data represent the mean ± SEM, *p < 0.5, **p < 0.01. Scale bar, 20 μm