Fig. 5

YAP and TAZ knockdown delayed erythroid maturation and enucleation. A Schematic of YAP knockdown during erythroid differentiation using the CRISPR/Cas9 PX458-GFP_gYAP plasmid. B YAP mRNA expression of PB- and CB-CD34⁺ HSC-derived erythroblasts at 3 days post-nucleofection, as analyzed by qRT-PCR (n = 4). C A representative of erythroid cell morphology during differentiation after YAP-KD showed delayed differentiation and more immature cells (black arrow) compared to control. D Percentages of cells after YAP-KD of PB- and CB-CD34⁺ HSCs counted at day 15 of differentiation. At least 200 cells were counted in each group (n = 4). E KLF1 mRNA expression of sorted GFP⁺ cells at 3 days after nucleofection (n = 3). F Schematic of TAZ knockdown during erythroid differentiation using CRISPR/Cas9 pLenti_gWWTR1 (TAZ). G TAZ mRNA expression at 3 days post-nucleofection (n = 3, 4, respectively). H Representative erythroid cell morphology derived from PB- and CB-CD34⁺ HSCs, after TAZ-KD. I Percentages of cells after TAZ-KD at day 15 of differentiation. At least 200 cells were counted in each group (n = 3, 4, respectively). J KLF1 mRNA expression after TAZ-KD analyzed 3 days after nucleofection (n = 3). Data represent the mean ± SEM. *p < 0.05, **p < 0.01. Scale bar, 20 μm