Fig. 6

Overexpression of YAP/TAZ had no apparent effect on erythroid differentiation. A Schematic of YAP and TAZ overexpression in erythroid cells using constitutively active YAP and TAZ plasmids named YAPS5A and TAZS89A, respectively. B YAP protein expression analyzed by Western blotting at 3 days post-nucleofection and relative expression to β-actin were labeled in red. C YAP mRNA expression as analyzed by qRT-PCR at 3 days post-nucleofection (n = 4). D TAZ protein expression as analyzed by Western blotting at 3 days post-nucleofection and relative expression to β-actin were labeled in red. E TAZ mRNA expression was analyzed by qRT-PCR at 3 days post-nucleofection (n = 5). F, G Representative cell morphology after YAP and TAZ overexpression. H Percentages of cells after YAP and TAZ overexpression at day 18 of differentiation. At least 200 cells were counted in each group (n = 3). Data represented in the mean ± SEM. *p < 0.05, **p < 0.01. Scale bar, 20 μm