Fig. 7

Knockdown of YAP or TAZ in CD34⁺ HSCs did not alter HSC commitment to erythroid or myeloid lineages. A Schematic of YAP and TAZ knockdown in CD34⁺ HSCs using CRISPR/Cas9 lentiviral system. B YAP and TAZ mRNA expression as analyzed by qRT-PCR at 2 days after lentiviral transduction (n = 4, 3 respectively). C Representative of colony-forming unit (CFU) assay of YAP-KD, TAZ-KD, and YAP/TAZ-double (d) KD in PB-CD34⁺ HSCs after being cultured in methylcellulose for 14 days showing colony-forming unit granulocyte/erythroid/macrophage/megakaryocyte (CFU-GEMM), colony-forming unit granulocyte/macrophage (CFU-GM) and Burst-forming unit erythroid (BFU-E). D The colony number of YAP-KD, TAZ-KD, and YAP/TAZ-dKD derived from PB-CD34⁺ HSCs counted on day 14 of methylcellulose culture (n = 3). E Percentage of erythroid cells (Ery) and granulocytes/macrophages (GM) after YAP-KD, TAZ-KD, and YAP/TAZ-dKD of cells from methylcellulose (n = 3). F Summary of this study. Data represent the mean ± SEM, *p < 0.05. Scale bar, 200 μM