Fig. 4

HAC-MSC-sensitized serum enhanced ADCC. DAPI-positive dead cells in the CD11b-negative fraction were collected after 24 h of co-culture of BMDMs and HAC-MSCs. M0: BMDM (medium only), M1: BMDM (LPS + IFN-γ stimulation 24 h), M2: BMDM (IL-4 stimulation 24 h), BMDM (−): no BMDM. Non-BMDM dead cell state for each BMDM-activated phenotype is shown (A). The t test between normal and sensitized sera within the corresponding BMDM activation phenotypes (B). Differences between normal and sensitized sera in M0 and BMDM (−) groups were observed. Data are expressed as the mean ± S.E. (n = 4, each phenotype). There were no significant differences among the sensitized groups (M0, M1, and M2) or normal groups